Liquid Chromatography – Mass Spectrometry (LC–MS/MS)

 Course Duration: 2 Hrs.  (Online)

• Liquid Chromatography–Mass Spectrometry (LC–MS/MS) training courses Class Room Link / Training Link will be shared through your registered email after the completion of the fee payment.
• Participants need to go through the Each chapter and complete the final assessment.
• Duration of the Program: (2 Hrs.) 
• Examination should be completed within One Month.  
• Course materials can be downloaded from the classroom.
• Upon completion of the course, participants gain the INTERNATIONAL CERTIFICATE

LC–MS/MS Self-Learning Course

Course Overview
This comprehensive self-paced course provides an in-depth understanding of Liquid Chromatography–Mass Spectrometry (LC–MS/MS) — from fundamental principles to advanced applications. Designed for scientists, analysts, and students in analytical chemistry, pharmaceuticals, food safety, and environmental sciences, this program blends theory with practical insights to help you confidently operate, troubleshoot, and optimize LC–MS/MS systems.

What You’ll Learn

1. Fundamentals of LC–MS

• Core concepts of LC–MS techniques and their wide-ranging applications.
• Overview of LC unit components and complete LC–MS system architecture. 
• Understanding LC chromatograms and detector types.
• Integration of LC with MS for advanced analytical workflows.
   

2. Liquid Chromatography Techniques

• Reversed-Phase LC (RPLC) – principles and applications.
• Normal-Phase LC (NPLC).
• Hydrophilic Interaction Liquid Chromatography (HILIC).
• Ion Exchange Chromatography.
• Size Exclusion Chromatography (SEC).
   

3. Interfaces & Ionization Techniques

• Role of LC–MS interfaces and their configurations.
• Electrospray Ionization (ESI).
• Atmospheric Pressure Chemical Ionization (APCI).
• Atmospheric Pressure Photoionization (APPI).
   

4. Mobile Phases & Additives

• Choosing mobile phases compatible with LC–MS.
• Volatile vs. non-volatile mobile phases.
• Recommended mobile phases for each ionization technique.
• Additive selection for improved signal and reduced noise.
• Matrix effect mitigation strategies.
   

5. Principles of Mass Spectrometry

• Fundamentals of MS detection and signal interpretation.
• Quadrupole MS principles, scan mode, and selected ion monitoring (SIM) mode.
• Tandem MS (MS/MS) systems and collision cell fragmentation processes.
• Types of scan and monitoring modes in MS/MS.
• Triple Quadrupole (TQ) MS and other MS/MS system types.
   

6. Sample Preparation for LC–MS/MS

• Solid Phase Extraction (SPE) — principles and workflows.
• Protein Precipitation techniques for biological samples.
• Liquid–Liquid Extraction (LLE) methods for diverse matrices.
   

Key Features

• Self-paced learning modules with clear illustrations.
• Practical tips for method development and troubleshooting.
• Step-by-step workflows for sample preparation and analysis.
• Suitable for both beginners and experienced analysts.
   

Who Should Enroll

• Analytical chemists and laboratory technicians.
• Researchers in pharmaceuticals, food safety, forensics, and environmental testing.
• Students pursuing studies in chemistry, biochemistry, and related fields.
   

By the end of this course, you will have the knowledge and confidence to design, operate, and optimize LC–MS/MS methods for a variety of complex analytical challenges. 

Assessment: Participants may be evaluated through quizzes, and a final examination to ensure comprehension and application of the course material. 

Outcome: Participants completing the Liquid Chromatography–Mass Spectrometry (LC–MS/MS) Training Program will be equipped to plan, conduct, report, and follow up audits effectively. They will gain the INTERNATIONAL CERTIFICATE.

Course Duration: 

• SELF-PLACED COURSE (Do not Wait ! Start Now !)

SELF-PLACED COURSE ( Do not Wait! Start Now !)

• After fee payment we will share the Self-Placed Course Link to your registered email address.
• After receiving mail join the class and start learn and complete the assignment and examinations.
• U-TECH INTERNATIONAL certificate of “Liquid Chromatography–Mass Spectrometry (LC–MS/MS)” will be issued to participants only after successful completion based on written examination 

Training Link: Will be shared through your registered email after the completion of the fee payment.

• Participants will be assessed through a 50 marks MCQ type online examination (open book) at the end of the course
• Minimum passing% criteria: 60%.( 30 Marks)

• U-TECH certificate of “Liquid Chromatography–Mass Spectrometry (LC–MS/MS)l” will be issued to participants only after successful completion based on performance during the course and in written examination 
• Others who have not successfully completed the course will be issued a certificate of attendance.

Training Link: This will be shared through your registered email after the completion of the fee payment.

Course materials will be provided for all participants