Course Fees: 799 INR / 20 USD

Course Duration: 2 Hrs. (Online)

• Class Room Link / Training Link will be shared through your registered email after the completion of the fee payment.
• Participants need to go through the Each chapter and complete the final assessment.
• Total Chapter: 1 to 4
• Duration of the Program: (2 Hrs.) One Month 
• Examination should be completed within One Month.  
• Course materials can be downloaded from the classroom.
• Upon completion of the course, participants gain the INTERNATIONAL CERTIFICATE

Course Overview: This course is designed to provide participants with a comprehensive understanding of molecular biology techniques PCR & RT-PCR (Real Time - Polymerase Chain Reaction) based on international standard methods. Participants will gain practical knowledge and skills necessary to assess and manage molecular biological risks in molecular biology analysis, ensuring compliance with international standards.

Target Audience:

• Microbiologists
• Molecular biologist
• Medical Technologist
• Medical Laboratory Technicians
• Quality assurance professionals
• Biomedical Scientist
• Physician / Doctors 
• Regulatory affairs personnel
• Professionals involved in Clinical microbiology analysis

Course Modules:

Molecular Biology Techniques

• Biosafety Introduction
• Molecular laboratory working areas
• Preventing and eliminating contamination
• Nucleic Acid Extraction Overview
• Common Types of Nucleic Acid Extraction Methods
• Liquid-Phase Extraction methods
• Solid-phase extraction methods
• Method for the quantitation of extracted DNA: Basic ultraviolet spectrometric method.
• Gel electrophoresis 
• Special Precautions and Troubleshooting
• Troubleshooting: DNA Degradation
• Troubleshooting: RNA Degradation
• Troubleshooting: Low Nucleic Acid Yields
• Troubleshooting: Low Purity

Methods of analysis for the detection of genetically modified organisms

• Preparation of the test portion

DNA extraction/purification. Methods for DNA extraction

• Preparation of PCR-quality DNA using phenol/chloroform-based DNA extraction methods. 
• Preparation of PCR-quality DNA using poly vinyl-pyrrolidone (PVP)-based DNA extraction methods.
• Preparation of PCR-quality DNA using the CTAS-based DNA extraction methods (Basic CTAB method)
• Preparation of PCR quality DNA using the silica-based DNA extraction methods.
• Preparation of PCR quality DNA using guanidinium-chloroform-based DNA extraction methods

The detection of genetically modified organisms’ Qualitative nucleic acid-based methods

• Aspects of PCR design
• Primer design
• PCR set-up, detection, and confirmation of PCR products
• Screening method for the detection of genetically modified plant DNA (CaMV 35S promoter).
• Screening method for the detection of genetically modified plant DNA (Agrobactenum tumefaciens N OS-terminator)
• Screening method for the detection of genetically modified plant DNA (npt II gene)
• Interpretation and expression of results

Real- time PCR method for the qualitative of the extracted meat DNA

• Molecular biomarker analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Bovine DNA detection method
• Molecular Biomarker Analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Buffalo DNA detection method
• Molecular Biomarker Analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Chicken DNA detection method
• Molecular Biomarker Analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Porcine DNA detection method
• Molecular Biomarker Analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Ovine DNA detection method
• Molecular Biomarker Analysis - Detection of animal derived materials in foodstuffs and feedstuffs by real-time PCR —Goat DNA detection method
• Molecular Biomarker Analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Horse DNA detection method.
• Molecular Biomarker Analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR —Turkey DNA detection method
• Molecular Biomarker Analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR —Donkey DNA detection method
• Molecular biomarker analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Goose DNA detection method
• Inference (Qualitative Analysis)

Course material will be provided...

Assessment: Participants may be evaluated through quizzes, and a final examination to ensure comprehension and application of the course material.

Upon completion of the course, participants gain the INTERNATIONAL CERTIFICATE and should be equipped with the knowledge and skills required to implement and maintain molecular biological safety.

Training Link: This will be shared through your registered email after the completion of the fee payment.

Course materials will be provided for all participants